Synthesis of Replicative Form Deoxyribonucleic Acid and Messenger Ribonucleic Acid by Gene IV Mutants of Bacteriophage S13
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چکیده
منابع مشابه
Characterization of bacteriophage S13 suN15 single-strand and replicative-form deoxyribonucleic acid.
The deoxyribonucleic acid (DNA) of bacteriophage S13 was shown to be single-stranded by the criteria of reactivity with formaldehyde, dependence of optical density on ionic strength, broad temperature-absorbance profile, and lack of molar equivalence of the purine and pyrimidine bases. The DNA has a molecular weight of 1.8 x 10(6) daltons, an S degrees (20) of 24.6 in SSC (0.15 m NaCl plus 0.01...
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A detailed qualitative and quantitative comparison was made of the ultrastructure of single-stranded ribonucleic acid (RNA) from bacteriophage R17 and double-stranded replicative form (RF) and replicative intermediate (RI) from cells infected with this bacteriophage. The nucleic acids were prepared for electron microscopy by the protein monolayer spreading technique of Kleinschmidt. Single-stra...
متن کاملEnzymatic Synthesis of Ribonucleic Acid. Iv. the Deoxyribonucleic Acid-directed Synthesis of Complementary Cytoplasmic Ribonucleic Acid Components.
The ribonucleic acid product formed by ribonucleic acid polymerase resembles its deoxyribonucleic acid template in base composition and in base sequence (l-6). The reaction in vitro results in the synthesis of RNA chains which are complementary to both DNA strands (6). Evidence for this originates from the formation of highly ordered RNA structures when the RNA polymerase product is annealed wi...
متن کاملThe role of deoxyribonucleic acid in ribonucleic acid synthesis. IV. The incorporation of pyrimidine and purine analogues into ribonucleic acid.
The synthesis of ribonucleic acid from ribonucleoside triphosphates by an enzyme system dependent on deoxyribonucleic acid has been described (2-7). The base composition of the RNA produced is identical with that of the DNA which is added. This result can be attributed, by an analogy with the complementary base model of DNA structure proposed by Watson and Crick (8), to hydrogen bonding by succ...
متن کاملThe role of deoxyribonucleic acid in ribonucleic acid synthesis. III. The inhibition of the enzymatic synthesis of ribonucleic acid and deoxyribonucleic acid by actinomycin D and proflavin.
* Report of work supported by the U.S. Public Health Service, grants no. RG-3977, RG-5143, and A-3102. We wish to acknowledge the fine technical assistance of Hedi Imfeld and the counsel and cooperation of F. William Studier. 1 Huang, R. C., N. Maheshwari, and J. Bonner, Biochem. Biophys. Res. Comm., 3, 689 (1960). 2 Bonner, J., R. C. Huang, and N. Maheshwari, these PROCEEDINGS, 47, 1548 (1961)...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1969
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.4.4.394-399.1969